RESUMO
Soil-transmitted helminth infections remain a major public health burden in low- and middle-income countries. The traditional diagnosis by microscopic examination of fecal samples is insensitive and time-consuming. In this study, a pentaplex real-time polymerase chain reaction (PCR) was evaluated for the simultaneous detection of Ancylostoma, Necator americanus, Ascaris lumbricoides, and Strongyloides stercoralis. The results were compared with those obtained by conventional parasitological diagnostic methods. Real-time PCR was positive in 48 of 77 samples (62.3%) and microscopic examination was positive in six samples (7.8%) only (P < 0.05). In conclusion, the real-time PCR assay described in this study provides a specific and sensitive diagnostic tool for the detection of these four helminth species in epidemiological studies and monitoring of treatment programs.
Assuntos
Ancylostoma , Ancilostomíase/diagnóstico , Ascaríase/diagnóstico , Ascaris lumbricoides , Necator americanus , Necatoríase/diagnóstico , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Strongyloides stercoralis , Estrongiloidíase/diagnóstico , Ancylostoma/genética , Animais , Ascaris lumbricoides/genética , DNA de Protozoário/genética , Fezes/parasitologia , Necator americanus/genética , Contagem de Ovos de Parasitas , Strongyloides stercoralis/genéticaRESUMO
This study aims to elucidate the effects of chrysin on human ER-negative breast cancer cell line, MDA-MB-231. The study demonstrated that treatment of MDA-MB-231 cells with 20 microM chysin for 48 h significantly inhibited the growth of MDA-MB-231 cells and induced cytoplasmic lipid accumulation in the cells, but that the observed of cell death was not caused by apoptosis. The expression of PPARalpha mRNA in chrysin-treated MDA-MB-231 cells was significantly increased, which was likely associated to the proliferation of the cells post chrysin treatment.
